Seed germination in response to pH: Effect of tap water, alkaline water, and carbonated water on Phaseolus lunatus


  • Vanessa Chow
  • Julia Shim
  • Shannon Lo


This study aims to determine which type of water and what pH range is most optimal for
germinating seeds of Phaseolus lunatus, as there have been limited studies focused on the pH of
water. Seeds of P. lunatus were germinated under three separate water treatment groups with
varying pH values. In this particular study, we used tap water from British Columbia’s lower
mainland, alkaline water, and carbonated water. P. lunatus root growth was observed over the
span of two weeks, where we considered a longer root length to be associated with higher
germination success. We calculated a mean root length using the compiled data, which included
each group member's measurements. P. lunatus germinated in a tap water environment had the
longest mean root length of 9.83 centimeters; however, using a Kruskal-Wallis test, these results
were not found to be statistically significant (p= 0.9049). With this, we conclude that none of the
water treatments yielded P. lunatus root lengths that were statistically greater in length compared
to P. lunatus grown in other conditions.
Keywords: water, pH, acidic, alkaline, germination, Phaseolus lunatus, lima beans