Different Light Wavelengths and Oxygen Production in C hlamydomonas reinhardtii


  • Mathew Gian
  • Katie Kathiresan
  • Yvonne Nguyen
  • Carmela Sangalang


Chlamydomonas reinhardtii is a green alga widely distributed in soil and freshwater
systems that serves as a source of food and oxygen for salmon species. Our study examined the
effect of different light wavelengths on C. reinhardtii oxygen production from photosynthetic
activity. These different light wavelengths consisted of white light (control), red light, blue light,
and green light that were shone on six vials of C. reinhardtii culture. It was predicted that white
light would yield the highest concentration of oxygen while red light would produce the lowest
concentration. These six vials were subjected to similar luminance and temperature conditions
which were measured for oxygen concentration with a dissolved-oxygen probe connected to a
TI-84 calculator. A one-way ANOVA test comparing oxygen production between different light
treatment groups revealed there was a significant difference between the white light treatment
group and the red, blue and green treatment groups. The test also revealed there was a significant
difference between red and blue, red and green, and blue and green light oxygen production
based on Tukey’s multiple comparison test. The highest oxygen production was measured from
the green light treatment group, while the lowest oxygen production was found in the red light
treatment group.