Vol. 4 (2025): Undergraduate Journal of Experimental Microbiology and Immunology Methods (UJEMI Methods)

Methods Article

• Replacing the Kanamycin Resistance Cassette with a Chloramphenicol Resistance Cassette in Plasmids for Transformation into Kanamycin Resistant Bacterial Strains Including the Keio Collection Using a Modified Gibson Assembly

  Arian Afshari, Muhammed Atasoy, Ramdeep Kailay, Amy Kim, Sherin Xierenayi

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• Autoaggregation and Morphology Assay for Determining Functionality and Transport Efficiency of the Autotransporter Antigen 43 in Escherichia coli Strain DH5α

  Chloe Chan, Kaitlin Law, Michelle Tong, Anny Xia

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• Agarose Gel Electrophoresis for Analysis of PCR Products

  Chaeyoon Chang, Julia Jung, Aaron Kwai, Elijah Uy

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• Enhanced Specificity in Western Blot Detection of Antigen 43 in Escherichia coli

  Lily Chen, Manveer Dhillon, Shreya Gandhi, Tricia Santiago

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• An Optimized Approach to Measure Ag43-Mediated Autoaggregation in DH5α Escherichia coli

  QingRu Kong, Ran Tao, Keheng Wang, Zurui Zhu

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