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Abstract
The effects of arginine, lysine and ornithine on the transformation of plasmid pBR322 were tested against the known effects of CaCl on transformation in Escherichia coli strain 2 HB101. In the first experiment, four different sets of the strain were transformed, each using a different ion to induce transformation with 100 ng of plasmid present. The second time around, 12 different sets of the strain were transformed in groups of four, each with a different ion. The first group was transformed with 10 ng of plasmid, the second with 100 ng of plasmid, and the last with 500 ng of plasmid. The results showed that arginine and lysine could induce transformation but not as well as CaCl and they also showed that the more 2 plasmid present, the better the transformation frequency in the case of CaCl. 2