Effects of Tricine, Glycine and Tris Buffers on Alkaline Phosphatase Activity
Vol. 2 (2002), Research Article
Vol. 2 (2002)

Effects of Tricine, Glycine and Tris Buffers on Alkaline Phosphatase Activity

Research Article
https://doi.org/10.14288/ujemi.v2.204240
Published 2002-04-01
JAMES HETHEY
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
JACQUELINE LAI
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
SLADE LOUTET
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
MORGAN MARTIN
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
VINCENT TANG
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
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How to Cite

HETHEY, J., LAI, J., LOUTET, S., MARTIN, M., & TANG, V. (2002). Effects of Tricine, Glycine and Tris Buffers on Alkaline Phosphatase Activity. Undergraduate Journal of Experimental Microbiology and Immunology, 2, 33–38. https://doi.org/10.14288/ujemi.v2.204240
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Abstract

The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. Michaelis- Menten enzyme kinetic analysis revealed that the presence of Tris produced the highest V max and K values for alkaline phosphatase at each pH tested, while the presence of Glycine m produced the lowest. Also, increasing the pH was found to increase both the V and K. max m

https://doi.org/10.14288/ujemi.v2.204240
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