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Abstract
– The binding dynamics of bacteriophage T4 to wild type, OmpC and an LPS mutated strain of Escherichia coli were assessed. Previous authors determined that bacteriophage T4 was – inhibited from infecting the OmpC strain and showed decreased infectivity in the specific LPS mutants. To determine why infectivity rates for the mutants were decreased, we used a phage-binding assay to establish whether was limited due to a lack of bacteriophage binding or due to inhibition of injection of bacteriophage DNA into the cell. Unfortunately, due to unknown reasons, the binding assays were not successful. Secondary experiments were conducted to deduce the possible reasons for failure of the assay. Our results indicated that the failure might have been due to an insufficient multiplicity of infection or to too dense a lawn of indicator cells, which could inhibit plaque formation.