The Effect of Colloidal n-C60 on DNA Mutagenesis and the SOS DNA Repair Mechanism of Salmonella typhimurium strains TA1538 and TA1535.

Abstract

The Ames test was utilized to examine the effect of n-C60 on growth, mutagenicity, and DNA repair mechanisms. Nano-C60 was prepared using the THF solubilization method and tested against Gram-negative histidine mutant Salmonella typhimurium strains TA1538 and TA 1535, at concentrations of 0.04, 0.12, 0.21, 0.26, 0.43, 0.53 mg/L to test for effects on growth and mutagenicity. UV treatment at 118 Jm-2 was used to induce the SOS DNA repair response to investigate the effect of n-C60 on DNA repair mechanisms. No apparent growth inhibition was observed with both strains grown under varying concentrations of n-C60 and no mutagenicity was seen with TA1535, but the classification of n-C60 as a mutagen was inconclusive with TA1538 grown under the same conditions. Lack of growth on UV treated plates is likely due to a lethal UV dosage and therefore the effect of n-C60 on the SOS DNA repair response was not determined.