Assessing binding ability and stability of T4 bacteriophage to Escherichia coli with mutations in the T4 bacteriophage receptors

Abstract

The Ames test was utilized to examine the effect of n-C on growth, mutagenicity, and 60 DNA repair mechanisms. Nano-C was prepared using the THF solubilization method 60 and tested against Gram-negative histidine mutant Salmonella typhimurium strains TA1538 and TA 1535, at concentrations of 0.04, 0.12, 0.21, 0.26, 0.43, 0.53 mg/L to test for effects on -2 growth and mutagenicity. UV treatment at 118 Jm was used to induce the SOS DNA repair response to investigate the effect of n-C on DNA repair mechanisms. No apparent 60 growth inhibition was observed with both strains grown under varying concentrations of n- C and no mutagenicity was seen with TA1535, but the classification of n-C as a mutagen 60 60 was inconclusive with TA1538 grown under the same conditions. Lack of growth on UV treated plates is likely due to a lethal UV dosage and therefore the effect of n-C on the 60 SOS DNA repair response was not determined.